MK Dons Under-18s were beaten 3-1 by Leyton Orient in their opening Youth Alliance Merit League 2 fixture.
Tali Jallow put the Dons ahead just before the half-hour mark but three second-half goals from the hosts saw them claim all three points.
Next up for John Bitting’s side is a home fixture with Forest Green Rovers this weekend.
MK Dons: Hayton, Johnson, Kane (Boyce 75), Stirland (Wood 75), Scholtz, Waller, Lewis-Burgess (Bombo 70), Tripp (Adepojli 75), Blennerhassett, Daffern, Jallow.
Subs: None
Goal: Jallow
Booked: Stirland