Karl Robinson makes two changes to the side which lost to Shrewsbury Town on Tuesday night.
In to the team comes Patrick Bamford, who will occupy a position on the right-hand side of a front three, and Stephen Gleeson, who makes his first start since recovering from a broken foot – an injury which had kept him out of action for three months.
Gleeson will start in the ‘number 10’ role in between Bamford and Dean Bowditch on the left, while Ryan Lowe wins the battle for the solitary striker’s role after his brace in mid-week. Adam Chicksen is at right-back, in place of Mathias Kouo-Doumbe, who drops to the bench.
Elsewhere, Ian McLoughlin remains between the sticks while Jon Otsemobor, Alan Smith, Izale McLeod and George Baldock are available as substitutes.
Luke Chadwick, David Martin and Daniel Powell miss out through injury.
MK Dons (4-2-3-1): McLoughlin; Chicksen, A Kay, Williams, Lewington (c); Potter, Lines; Bamford, Gleeson, Bowditch; Lowe.
Subs: Otsemobor, Kouo-Doumbe, Smith, McLeod, Baldock.
Tranmere Rovers: Fon Williams (c), Holmes, Black, Taylor, Palmer, Harrison, Bakayogo, Power, Gibson, Sidibe, Akpa Akpro.
Subs: Mooney (GK), Stockton, Amoo, M Kay, O'Halloran, Bell-Baggie, Corry.
Oliver Langford (West Midlands)Referee: