Karl Robinson hands Dele Alli his first start for the Club, after the youngster impressed on his professional debut in the original fixture.
The 16-year-old will partner Stephen Gleeson in midfield in the absence of Darren Potter who has a sore Achilles.
Elsewhere, there are three changes to the side that beat Sheffield United on Saturday with Mathias Kouo-Doumbe, Jay O'Shea and Charlie MacDonald coming in for Jon Otsemobor, Angelo Balanta and Luke Chadwick.
Antony Kay and last weekend’s goal hero Shaun Williams continue their partnership at the back in front of ‘keeper David Martin while Ryan Lowe retains his place up top for the Dons.
Academy prospects Mason Spence, Brendan Galloway and Giorgio Rasulo are all handed places on the substitute’s bench alongside Ian McLoughlin, Adam Chicksen and Alan Smith.
MK Dons (4-2-3-1): Martin; Kouo-Doumbe, Kay, Williams, Lewington (c); Gleeson, Alli; Bowditch, MacDonald, O'Shea; Lowe.
Subs: McLoughlin, Smith, Chicksen, Galloway, Spence, Rasulo.
Cambridge City (4-4-2): Barrett; Pepper, Theobald, Chaffey, Brighton; Allen, Cambridge (c), Prada, Marriott; Bryant, Hammond.
Subs: Ordonez (GK), Abbs, Midgley, Nightingale, Lester, Stynes.
Referee: N Miller (County Durham)