Goals from Alan Sheehan, Kazenga LuaLua and Jorge Grant gave Luton Town a win on Matchday Two in the Checkatrade Trophy.
Paul Tisdale made eight changes to the starting XI that overcame Cheltenham Town 3-0 on Saturday with Dean Lewington, Ryan Watson and Chuks Aneke keeping their place from the win.
Finn Tapp, Oran Jackson and David Kasumu were all handed starts, while Mitch Hancox also earned 90-minutes.
If they are to have any chance of qualifying into the knockout stages - they'll have to beat Brighton Under-21s in the final Group Stage game.
It was the home side who had the first chance of the game as Jorge Grant’s floated cross found Andrew Shinnie on the edge of the box – the Luton midfielder opted to volley first time only to fire over.
Shinne was once again involved soon after as he missed the target from close range after Kazenga Lua Lua created space for the cross on the left.
Stuart Moore was forced to make his first save of the game with seven minutes on the clock, he got well down to his right to deny Arthur Read the opening goal of the game.
It was Ousseynou Cisse who had the first shot on goal for MK Dons with the Malian firing narrowly over from 30-yards.
With 13-minutes on the clock, Aaron Jarvis was presented with the best chance of the first half. It was created by right-back James Justin who floated a cross towards the Luton striker, Moore, however, was on hand to deny the Hatters an early goal.
Luton’s were soon given their best chance to take the lead when David Kasumu fouled Shinnie inside the box leaving Craig Hicks with no other choice than to award a penalty. Alan Sheehan stepped up to the spot and put his penalty straight down the middle. b
The Dons almost drew level immediately after and Kasumu headed Hancox’s cross back towards Cisse – the midfielder’s shot was saved by Isted.
The pressure continued when Lewington and Simpson combined well on the edge of the box before Aneke’s curling strike was claimed by the Luton shot-stopper.
Luton soon doubled their advantage after 28-minutes with their goal coming from a corner. The Hatters went short to LuaLua who cut inside before curling past Moore into the back of the net. 2-0.
Watson had the final chance of the opening 45, his shot from the edge of the box failed to trouble Isted.
Paul Tisdale used the half-time break to rotate his senior players with Alex Gilbey, Aidan Nesbitt and Brandon Thomas-Asante replacing Chuks Aneke, Dean Lewington and Ouss Cisse respectively.
It was almost an immediate impact for Nesbitt with the forward going close twice within a matter of minutes. Firstly, his right-footed strike was saved by Isted down to his right before the Luton keeper produced two stunning saves from close range to deny to Scot his first goal of the season.
Moore, who had already made a handful of impressive saves, was on hand to deny Shinnie before Read headed wide from 6 yards.
Luton bagged their third of the afternoon on the hour mark thanks to the awareness of Jorge Grant. Moore got down well to deny Jarvis only for the Luton striker to convert from close range. 3-0.
Moore and Tapp both denied Luton a fourth of a game, the latter producing a late tackle to block Read's effort when as Luton man was one-on-one with Moore.
Moore continued to impress between the sticks when he was on hand to save from LuaLua when the winger looked certain to score. He then saved from James Justin’s effort from the edge of the not. Dons continued to look for a goal and it almost came via Nesbitt. Simpson headed into the path of the substitute – his half-volley ended just wide.
Starting XI: Moore, Hancox, Tapp, Jackson, Brittain; Lewington (Gilbey, 46), Cisse (Nesbitt, 46), Watson, Kasumu; Aneke (Thomas-Asante, 46), Simpson.
Unused subs: Sietsma, Nombe, Sole, Pattison
Booked: Aneke, Hancox.
Attendance: 875 (208)
Referee: C Hicks.